pRS414-7x2-PHO5-GFP-hPLC delta PH domain dimer
(Plasmid
#58837)
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PurposeExpresses GFP-Tagged Human PLC delta PH domain in yeast
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS414
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Backbone manufacturerStratagene
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name2x Phospolipase C delta1 PH domain
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Alt namePLCD1
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SpeciesR. norvegicus (rat)
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GenBank IDNM_001130964.1
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Entrez GenePlcd1 (a.k.a. Plc1)
- Promoter PHO5
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PH domain of PLC delta= Methionine 1 - Glutamine 175
This plasmid contains two copies of hPLC delta PH domain in tandem.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS414-7x2-PHO5-GFP-hPLC delta PH domain dimer was a gift from Tim Levine (Addgene plasmid # 58837 ; http://n2t.net/addgene:58837 ; RRID:Addgene_58837)