pRS416-PHO5-GFP-Opi1 + terminator
(Plasmid
#58836)
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PurposeExpresses GFP-Tagged Opi1 + Terminator in yeast
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58836 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS416
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Backbone manufacturerStratagene
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOpi1p
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Alt nameYHL020C
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SpeciesS. cerevisiae (budding yeast)
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GenBank IDNM_001179100.1
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Entrez GeneOPI1 (a.k.a. YHL020C)
- Promoter PHO5
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid includes the Opi1p gene's own terminator.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS416-PHO5-GFP-Opi1 + terminator was a gift from Tim Levine (Addgene plasmid # 58836 ; http://n2t.net/addgene:58836 ; RRID:Addgene_58836)