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Addgene

pSil-ETN-ires-GFP
(Plasmid #58831)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 58831 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSil
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ETN version of Rabies glycoprotein external domain fused to normal Rabies glycoprotein cellular domain
  • Species
    rabies virus
  • Insert Size (bp)
    1575
  • Promoter CAG
  • Tag / Fusion Protein
    • ires-EGFP (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ggagggccttcgtgcgt
  • 3′ sequencing primer CTCAAGGGGCTTCATGATG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The ETN-rabies glycoprotein template was from Ian Wickersham

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSil-ETN-ires-GFP was a gift from Troy Margrie (Addgene plasmid # 58831 ; http://n2t.net/addgene:58831 ; RRID:Addgene_58831)
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