pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation Sequences (3)

Partial Sequences from Addgene (3)

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  > M13pUC-Rev 5'-AGCGGATAACAATTTCACACAGG-3' AGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTGGCGGCCCCCCCTCGAG GTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCAC CGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACC ATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGG CGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGAT CGATCCGGTCACCTTACTTGGCAAGGCATATACCCATTTGGGATAAGGGTAAACATCTTTGAATTGTCGA AATGAAACGTATATAAGCGCTGATGTTTTGCTAAGTCGAGGTTAGTATGGCTTCATCTCTCATGAGAATA AGAACAACAACAAATAGAGCAAGCAAATTCGAGAATTCCTGCAGCCCGGGCAATTCGAATATAATGTCTA AAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAA ATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACT ACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTTACGTATGGTGTTCAATGCTTTTCAA GATACCCAGATCATATGAAACGGCATGAC
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  > pGEX3 5'-CCGGGAGCTGCATGTGTCAGAGG-3' GGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATaaTAATGGTTTCTTAGACGGATCGCTTG CCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTA TTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAA AAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAA GCAGATTAAATAGATATACATTCGATTAACGATAAGTAAAATGTAAAATCACAGGATTTTCGTGTGTGGT CTTCTACACAGACAAGATGAAACAATTCGGCATTAATACCTGAGAGCAGGAAGAGCAAGATAAAAGGTAG TATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCT TTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCAC GTGATGAAAAGGACCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTA AATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTT TTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACAT CGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAG
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  > M13pUC-Fwd 5'-CCCAGTCACGACGTTGTAAAACG-3' GACTCACTATAGGGCGAATTGGGTACCAGAGCTCTTAAAGCTCATCATGTTTGTATAGTTCATCCATGCC ATGTGTAATCCCAGCAGCTGTTACAAACTCAAGAAGGACCATGTGGTCTCTCTTTTCGTTGGGATCTTTC GAAAGGGCAGATTGTGTGGACAGGTAATGGTTGTCTGGTAAAAGGACAGGGCCATCGCCAATTGGAGTAT TTTGTTGATAATGATCAGCGAGTTGCACGCCGCCGTCTTCGATGTTGTGGCGGGTCTTGAAGTTGGCTTT GATGCCGTTCTTTTGCTTGTCGGCCATGATGTATACGTTGTGGGAGTTGTAGTTGTATTCCAACTTGTGG CCGAGGATGTTTCCGTCCTCCTTGAAATCGATTCCCTTAAGCTCGATCCTGTTGACGAGGGTGTCTCCCT CAAACTTGACTTCAGCACGTGTCTTGTAGTTCCCGTCGTCCTTGAAGAAGATGGTCCTCTCCTGCACGTA TCCCTCAGGCATGGCGCTCTTGAAGAAGTCGTGCCGCTTCATATGATCAGGGTAACGGGAGAAGCACTGC ACGCCGTAGGTCAGGGTGGTGACCAGGGTTGGCCATGGAACAGGTAGTTTTCCAGTAGTGCAAATAAATT TAAGGGTAAGTTTTCCGTATGTTGCATCACCTTCACCCTCTCCACTGACAGAAAATTTGTGCCCATTAAC ATCACCATCTAATTCAACAAGAATTGGGACAACTCCAGTGAAAAGTTCTTCTCCTTTACTCATTTTTTCT ACCGGTAGAGATCCTTGTGTTTTGGAATGCGATATCAGGGCTGGAGATTTGTTCTTCAAGGCCAATGATG GTGGTACTGCC