pRS406-PHO5-GFP-2xOsh2 PH domain [short]
(Plasmid #58829)

• Purpose: Expresses GFP-Tagged Osh2 PH domain in yeast
• Depositing Lab: Tim Levine
• Publication: Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22.

Backbone

• Vector backbone: pRS406
• Backbone manufacturer: Stratagene
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 2xOsh2 PH domain [short]
• Alt name: Osh2p
• Species: S. cerevisiae (budding yeast)
• GenBank ID: NM_001180078.1
• Entrez Gene: OSH2 (a.k.a. YDL019C)
• Promoter: PHO5
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains two copies of the short form of Osh2 PH domain (short form: Serine286-Lysine39)