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Purpose(Empty Backbone) contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 58807 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC119
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Backbone manufacturerMessing Lab
- Backbone size (bp) 3345
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
All restriction sites between EcoRI and HindIII are single-cut except AscI. Multiple gRNA expression cassettes cloned within the MCSs between two AscI sites can be subcloned into the AscI site of pFGC-pcoCas9 binary plasmid (Addgene plasmid#52256) through one-step cloning.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC119-MCS was a gift from Jen Sheen (Addgene plasmid # 58807 ; http://n2t.net/addgene:58807 ; RRID:Addgene_58807)
Map uploaded by the depositor.
