pAAV-CaMKII-Chronos-GFP
(Plasmid #58805)

• Purpose: AAV expression of Chronos-GFP under the CaMKII promoter
• Depositing Lab: Edward Boyden
• Publication: Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 2014 Feb 9.

Backbone

• Vector backbone: AAV
• Backbone size w/o insert (bp): 5087
• Total vector size (bp): 6797
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Chronos-GFP
• Alt name: ChR90-GFP
• Alt name: Stigeoclonium helveticum channelrhodopsin
• Species: Stigeoclonium helveticum
• Insert Size (bp): 1710
• Promoter: CaMKII
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TCTCCATTTGCACTCAGG
• 3′ sequencing primer: cagcgtatccacatagcg

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is sequenced completely by the depositing lab except for parts of both ITRs. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro. They are 10 traditional basepair differences in the CamkII promoter relative to wild-type, which is not a problem.