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Addgene

p(+)MV-NSE-FlagP-F497A-3p Notes

Plasmid 58796 digest

These results confirm the presence of the unique AatII site noted as a backbone modification. The plasmid was digested with NotI and AatII as single and double digests. Linearized plasmid is 19.0 kb. NotI/AatII fragments are 6.3kb & 12.75kb.