pSplit-ON-hDib1
(Plasmid #58787)

• Purpose: Express hDib1-LucN fusion protein in mammalian cells
• Depositing Lab: Hiroyoshi Ariga
• Publication: Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 10.1016/j.ab.2014.01.015.

Backbone

• Vector backbone: pcDNA3
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hDib1
• Species: H. sapiens (human)
• Insert Size (bp): 429
• Entrez Gene: TXNL4A (a.k.a. BMKS, DIB1, DIM1, SNRNP15, TXNL4, U5-15kD)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Luciferase (1-398aa) (C terminal on backbone)
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry