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Addgene

JMC065-G11
(Plasmid #58762)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 58762 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    SGC
  • Vector type
    Baculovirus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDB:4PXW
  • Alt name
    DCAF1:JMC065-G11:C226091
  • Alt name
    BC110371
  • Alt name
    DCAF1
  • Species
    H. sapiens (human)
  • Entrez Gene
    DCAF1 (a.k.a. RIP, VPRBP)
  • Promoter polyhedrin promoter
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer polyhedrin_fwd_primer
  • 3′ sequencing primer SV40_PA_terminator
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JMC065-G11 was a gift from Cheryl Arrowsmith (Addgene plasmid # 58762 ; http://n2t.net/addgene:58762 ; RRID:Addgene_58762)
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