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Addgene

JMC034-H11
(Plasmid #58755)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58755 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28-MHL
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7325
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDB:4NW2
  • Alt name
    CHD1:JMC034-H11:C216622
  • Alt name
    BC117134/gi|68299794
  • Alt name
    PDB ID: 4NW2, 4O42
  • Species
    H. sapiens (human)
  • Mutation
    Bacterial expression for structure determination; may not be full ORF, see comments
  • Entrez Gene
    CHD1 (a.k.a. CHD-1, PILBOS)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JMC034-H11 was a gift from Cheryl Arrowsmith (Addgene plasmid # 58755 ; http://n2t.net/addgene:58755 ; RRID:Addgene_58755)
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