JMC034-H11
(Plasmid
#58755)
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Purposebacterial expression of PDB 4NW2
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58755 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerSGC
- Backbone size w/o insert (bp) 7325
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePDB:4NW2
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Alt nameCHD1:JMC034-H11:C216622
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Alt nameBC117134/gi|68299794
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Alt namePDB ID: 4NW2, 4O42
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SpeciesH. sapiens (human)
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MutationBacterial expression for structure determination; may not be full ORF, see comments
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Entrez GeneCHD1 (a.k.a. CHD-1, PILBOS)
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JMC034-H11 was a gift from Cheryl Arrowsmith (Addgene plasmid # 58755 ; http://n2t.net/addgene:58755 ; RRID:Addgene_58755)