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Addgene

JMC02A-C10
(Plasmid #58754)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58754 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28-MHL
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7325
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ALKBH5
  • Alt name
    ALKBH5:JMC02A-C10:C229582
  • Alt name
    BC062339
  • Alt name
    PDB:4O61, 4OCT
  • Species
    H. sapiens (human)
  • Mutation
    Bacterial expression for structure determination; may not be full ORF, see comments
  • Entrez Gene
    ALKBH5 (a.k.a. ABH5, OFOXD, OFOXD1)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JMC02A-C10 was a gift from Cheryl Arrowsmith (Addgene plasmid # 58754 ; http://n2t.net/addgene:58754 ; RRID:Addgene_58754)