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Addgene

pUltra-puro-RTTA3
(Plasmid #58750)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58750 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUltra-Chili
  • Backbone manufacturer
    Moore Lab, Addgene plasmid 48687
  • Modifications to backbone
    dtomato was replaced with puro
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Reverse TET-Transactivator 3
  • Alt name
    RTTA3
  • Mutation
    loxP5171
  • Promoter Ubiquitin C promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer hUBCpro-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RTTA3 was amplified from pSLIK-Neo-TGmiR-luc (Addgene plasmid 25743).

loxP5171 at positions after cppt and before WPRE

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUltra-puro-RTTA3 was a gift from Yildirim Dogan & Kitai Kim (Addgene plasmid # 58750 ; http://n2t.net/addgene:58750 ; RRID:Addgene_58750)
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