pKvenus-Scribble
(Plasmid #58738)

• Purpose: Expresses Venus-tagged human Scribble protein
• Depositing Lab: Ian Macara
• Publication: Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-8.

Backbone

• Vector backbone: pEGFPC1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 9624
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Scribble
• Alt name: SCRIB
• Species: H. sapiens (human)
• Insert Size (bp): 4893
• GenBank ID: NM_015356
• Entrez Gene: SCRIB (a.k.a. CRIB1, SCRB1, SCRIB1, Vartul, oSCRIB)
• Promoter: CMV
• Tag / Fusion Protein:
  • Venus (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: EGFP reverse

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A partial cDNA to human SCRIB was provided by Jon Huibregtse (Univ. of Texas, Austin TX). To generate a full length ORF, we amplified the 5' and 3' ends of the complete open reading frame by PCR from a human kidney cDNA library and ligated them into the partial clone.