Rabbit Cav 2.2 W391A pMT2
(Plasmid
#58734)
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Purposeexpression of rabbit CaV2.2 calcium channels with a mutation (W391) in the α1-interacting domain (AID) in the I-II loop of CaV2.2
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58734 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMT2
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCav2.2
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Alt namevoltage-dependent N-type calcium channel subunit alpha-1B
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SpeciesO. cuniculus (rabbit)
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MutationW391A
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GenBank IDD14157.1
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Entrez GeneCACNA1B
- Promoter SV40
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pMT2-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Original Cav2.2 plasmid from Yasuo Mori.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rabbit Cav 2.2 W391A pMT2 was a gift from Annette Dolphin (Addgene plasmid # 58734 ; http://n2t.net/addgene:58734 ; RRID:Addgene_58734) -
For your References section:
Interaction via a key tryptophan in the I-II linker of N-type calcium channels is required for beta1 but not for palmitoylated beta2, implicating an additional binding site in the regulation of channel voltage-dependent properties. Leroy J, Richards MW, Butcher AJ, Nieto-Rostro M, Pratt WS, Davies A, Dolphin AC. J Neurosci. 2005 Jul 27;25(30):6984-96. 10.1523/JNEUROSCI.1137-05.2005 PubMed 16049174