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Addgene

Rabbit Cav 2.2 W391A pMT2
(Plasmid #58734)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58734 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMT2
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cav2.2
  • Alt name
    voltage-dependent N-type calcium channel subunit alpha-1B
  • Species
    O. cuniculus (rabbit)
  • Mutation
    W391A
  • GenBank ID
    D14157.1
  • Entrez Gene
    CACNA1B
  • Promoter SV40

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Original Cav2.2 plasmid from Yasuo Mori.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rabbit Cav 2.2 W391A pMT2 was a gift from Annette Dolphin (Addgene plasmid # 58734 ; http://n2t.net/addgene:58734 ; RRID:Addgene_58734)
  • For your References section:

    Interaction via a key tryptophan in the I-II linker of N-type calcium channels is required for beta1 but not for palmitoylated beta2, implicating an additional binding site in the regulation of channel voltage-dependent properties. Leroy J, Richards MW, Butcher AJ, Nieto-Rostro M, Pratt WS, Davies A, Dolphin AC. J Neurosci. 2005 Jul 27;25(30):6984-96. 10.1523/JNEUROSCI.1137-05.2005 PubMed 16049174