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Mouse Alpha2 delta-2 mutMIDAS HA pMT2
(Plasmid #58733)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58733 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT2
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    alpha2 delta-2
  • Alt name
    Cacna2d2
  • Alt name
    calcium channel, voltage-dependent, alpha 2/delta subunit 2
  • Species
    M. musculus (mouse)
  • Mutation
    MIDAS site mutations (D300VSGS to AVAGA)
  • Entrez Gene
    Cacna2d2 (a.k.a. Cacna2d, a2d2, du, mKIAA0558, td, torpid)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pMT2-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is an HA tag in middle of insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse Alpha2 delta-2 mutMIDAS HA pMT2 was a gift from Annette Dolphin (Addgene plasmid # 58733 ; http://n2t.net/addgene:58733 ; RRID:Addgene_58733)
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