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Purposeexpression of HA tagged mouse Alpha2 delta-2 in mamalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58731 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMT2
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namealpha2 delta-2
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Alt nameCacna2d2
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Alt namecalcium channel, voltage-dependent, alpha 2/delta subunit 2
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SpeciesM. musculus (mouse)
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Entrez GeneCacna2d2 (a.k.a. Cacna2d, a2d2, du, mKIAA0558, td, torpid)
- Promoter SV40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pMT2-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is an HA tag in middle of insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mouse Alpha2 delta-2 HA pMT2 was a gift from Annette Dolphin (Addgene plasmid # 58731 ; http://n2t.net/addgene:58731 ; RRID:Addgene_58731) -
For your References section:
The calcium channel alpha2delta-2 subunit partitions with CaV2.1 into lipid rafts in cerebellum: implications for localization and function. Davies A, Douglas L, Hendrich J, Wratten J, Tran Van Minh A, Foucault I, Koch D, Pratt WS, Saibil HR, Dolphin AC. J Neurosci. 2006 Aug 23;26(34):8748-57. 10.1523/JNEUROSCI.2764-06.2006 PubMed 16928863