Mouse Alpha2 delta-3 HA pMT2
(Plasmid
#58728)
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Purposeexpression of HA tagged mouse Alpha2 delta-3 in mamalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58728 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMT2
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namealpha2 delta-3
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Alt nameCacna2d3
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Alt namecalcium channel, voltage-dependent, alpha2/delta subunit 3
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SpeciesM. musculus (mouse)
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Entrez GeneCacna2d3 (a.k.a. Cacnad3)
- Promoter SV40
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pMT2-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is an HA tag in middle of insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mouse Alpha2 delta-3 HA pMT2 was a gift from Annette Dolphin (Addgene plasmid # 58728 ; http://n2t.net/addgene:58728 ; RRID:Addgene_58728) -
For your References section:
The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a posttranslational modification essential for function. Davies A, Kadurin I, Alvarez-Laviada A, Douglas L, Nieto-Rostro M, Bauer CS, Pratt WS, Dolphin AC. Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1654-9. doi: 10.1073/pnas.0908735107. Epub 2010 Jan 4. 10.1073/pnas.0908735107 PubMed 20080692