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Addgene

Rat Alpha2 delta-1 pMT2
(Plasmid #58726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58726 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT2
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    alpha2 delta-1
  • Alt name
    Cacna2d1
  • Alt name
    calcium channel, voltage-dependent, alpha2/delta subunit 1
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Cacna2d1 (a.k.a. CCHLA2, Cacna2, DHSCCA)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pMT2-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rat Alpha2 delta-1 pMT2 was a gift from Annette Dolphin (Addgene plasmid # 58726 ; http://n2t.net/addgene:58726 ; RRID:Addgene_58726)

  • For your References section:

    The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a posttranslational modification essential for function. Davies A, Kadurin I, Alvarez-Laviada A, Douglas L, Nieto-Rostro M, Bauer CS, Pratt WS, Dolphin AC. Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1654-9. doi: 10.1073/pnas.0908735107. Epub 2010 Jan 4. 10.1073/pnas.0908735107 PubMed 20080692
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