pRS406-PHO5-GFP-CERT PH domain
(Plasmid
#58725)
-
PurposeExpresses GFP-Tagged CERT PH domain in yeast
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58725 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepRS406
-
Backbone manufacturerStratagene
-
Vector typeYeast Expression
-
Selectable markersURA3
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namecollagen, type IV, alpha 3 binding protein PH domain
-
Alt nameCERT
-
Alt nameGPBP
-
SpeciesH. sapiens (human)
-
GenBank IDNM_001130105.1
-
Entrez GeneCERT1 (a.k.a. CERT, CERTL, COL4A3BP, GPBP, MRD34, STARD11)
- Promoter PHO5
-
Tags
/ Fusion Proteins
- GFP (N terminal on backbone)
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PH domain of CERT= Methionine 1 - Glycine 122
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRS406-PHO5-GFP-CERT PH domain was a gift from Tim Levine (Addgene plasmid # 58725 ; http://n2t.net/addgene:58725 ; RRID:Addgene_58725) -
For your References section:
Targeting of Golgi-specific pleckstrin homology domains involves both PtdIns 4-kinase-dependent and -independent components. Levine TP, Munro S. Curr Biol. 2002 Apr 30;12(9):695-704. S0960982202007790 [pii] PubMed 12007412