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PurposeExpresses GFP-Tagged human FAPP1 PH domain in yeast
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58723 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS406
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Backbone manufacturerStratagene
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFour-phosphate-adaptor protein 1 PH domain
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Alt nameFAPP 1
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Alt namePLEKHA3
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SpeciesH. sapiens (human)
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Entrez GenePLEKHA3 (a.k.a. FAPP1)
- Promoter PHO5
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Tags
/ Fusion Proteins
- GFP (N terminal on backbone)
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PH domain of FAPP1= Methionine 1 - Threonine 98
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS406-PHO5-GFP-hFAPP1 PH domain was a gift from Tim Levine (Addgene plasmid # 58723 ; http://n2t.net/addgene:58723 ; RRID:Addgene_58723) -
For your References section:
Targeting of Golgi-specific pleckstrin homology domains involves both PtdIns 4-kinase-dependent and -independent components. Levine TP, Munro S. Curr Biol. 2002 Apr 30;12(9):695-704. S0960982202007790 [pii] PubMed 12007412