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Addgene

pET-20b-T7-Ntx-K49
(Plasmid #58715)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58715 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-20b(+)
  • Backbone manufacturer
    Novagen (EMD Millipore)
  • Backbone size w/o insert (bp) 3716
  • Total vector size (bp) 4035
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Notexin
  • Alt name
    P00608
  • Species
    Synthetic; Notechis scutatus scutatus
  • Insert Size (bp)
    402
  • Mutation
    Changed Aspartic Acid 49 to Lysine
  • Promoter T7
  • Tag / Fusion Protein
    • T7 peptide tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-20b-T7-Ntx-K49 was a gift from Fiorella Tonello (Addgene plasmid # 58715 ; http://n2t.net/addgene:58715 ; RRID:Addgene_58715)

  • For your References section:

    Production in Escherichia coli, folding, purification and characterization of notexin with wild type sequence and with N-terminal and catalytic site mutations. Simonato M, Morbiato L, Zorzi V, Caccin P, Fernandez J, Massimino ML, Polverino de Laureto P, Tonello F. Toxicon. 2014 Jun 18. pii: S0041-0101(14)00171-8. doi: 10.1016/j.toxicon.2014.06.009. 10.1016/j.toxicon.2014.06.009 PubMed 24951874
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