pET28a-ybbR-HIS-10FNIII(x4)-DocI
(Plasmid
#58712)
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PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5216
- Total vector size (bp) 6734
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name10FNIII(x4)-DocI
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Alt namefibronectin repeat 10 type III
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Alt namedockerin type I
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SpeciesSynthetic; Clostridium thermocellum
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Insert Size (bp)1050
- Promoter T7
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Tags
/ Fusion Proteins
- ybbR (N terminal on insert)
- HIS (N terminal on insert)
- HRV 3C (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWe thankfully received the dockerin domain from Professor Edward Bayer (Department of Biological Chemistry, Weizmann Institute of Science, Israel) as recently described in this paper: Stahl, S. W., Nash, M. A., Fried, D. B., Slutzki, M., Barak, Y., Bayer, E. A., & Gaub, H. E. (2012). Single-molecule dissection of the high-affinity cohesin-dockerin complex. Proceedings of the National Academy of Sciences of the United States of America, 109(50), 20431–6. doi:10.1073/pnas.1211929109
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-ybbR-HIS-10FNIII(x4)-DocI was a gift from Hermann Gaub (Addgene plasmid # 58712 ; http://n2t.net/addgene:58712 ; RRID:Addgene_58712) -
For your References section:
From genes to protein mechanics on a chip. Otten M, Ott W, Jobst MA, Milles LF, Verdorfer T, Pippig DA, Nash MA, Gaub HE. Nat Methods. 2014 Sep 7. doi: 10.1038/nmeth.3099. 10.1038/nmeth.3099 PubMed 25194847