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Addgene

pGEX mPtch-Cterm
(Plasmid #58704)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58704 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX
  • Backbone manufacturer
    Amersham
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ptch
  • Alt name
    Patched
  • Species
    M. musculus (mouse)
  • Mutation
    contains only Cterminus (aa ~1238-1413)
  • Entrez Gene
    Ptch1 (a.k.a. A230106A15Rik, Ptc, Ptc1, Ptch, mes, wig)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See antibody production protocol in supplemental documents.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX mPtch-Cterm was a gift from Matthew Scott (Addgene plasmid # 58704 ; http://n2t.net/addgene:58704 ; RRID:Addgene_58704)

  • For your References section:

    Patched1 regulates hedgehog signaling at the primary cilium. Rohatgi R, Milenkovic L, Scott MP. Science. 2007 Jul 20;317(5836):372-6. 10.1126/science.1139740 PubMed 17641202
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