pET28 mSmo-Cterm
(Plasmid
#58703)
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Purposeused for anti-mouse Smoothened antibody production (for affinity purification)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58703 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5369
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSmo
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Alt nameSmoothened
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SpeciesM. musculus (mouse)
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Mutationcontains C terminus (aa 543-793)
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Entrez GeneSmo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
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Tag
/ Fusion Protein
- 6X-HIS (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28 mSmo-Cterm was a gift from Matthew Scott (Addgene plasmid # 58703 ; http://n2t.net/addgene:58703 ; RRID:Addgene_58703) -
For your References section:
Patched1 regulates hedgehog signaling at the primary cilium. Rohatgi R, Milenkovic L, Scott MP. Science. 2007 Jul 20;317(5836):372-6. 10.1126/science.1139740 PubMed 17641202