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Addgene

pBluescript mouse ptc
(Plasmid #58699)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58699 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II KS+
  • Backbone manufacturer
    Stratagene
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ptc
  • Alt name
    Ptch1
  • Alt name
    patched homolog 1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ptch1 (a.k.a. A230106A15Rik, Ptc, Ptc1, Ptch, mes, wig)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This cDNA lacks a 5' and 3'UTR but has a Kozak consensus sequence for the ATG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript mouse ptc was a gift from Matthew Scott (Addgene plasmid # 58699 ; http://n2t.net/addgene:58699 ; RRID:Addgene_58699)
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