pcDNA3-calx1
(Plasmid
#58697)
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PurposeExpression of calx1
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecalx1
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Alt nameNa/Ca Exchanger Isoform 1
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SpeciesD. melanogaster (fly)
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Insert Size (bp)6860
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GenBank IDNM_079699
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Entrez GeneCalx (a.k.a. Dmel_CG5685, 9C5, CALX, CALX1.1, CG5685, CalX, Dmel/Ncx, Dmel\CG5685, NCX, Ncx, anon-93ABa, calX, calx)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-calx1 was a gift from Craig Montell (Addgene plasmid # 58697 ; http://n2t.net/addgene:58697 ; RRID:Addgene_58697) -
For your References section:
Light Activation, Adaptation, and Cell Survival Functions of the Na(+)/Ca(2+) Exchanger CalX. Wang T, Xu H, Oberwinkler J, Gu Y, Hardie RC, Montell C. Neuron 2005 Feb 3;45(3):367-78. 10.1016/j.neuron.2004.12.046 PubMed 15694324