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Addgene

pcDNA3-calx1
(Plasmid #58697)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58697 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    calx1
  • Alt name
    Na/Ca Exchanger Isoform 1
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    6860
  • GenBank ID
    NM_079699
  • Entrez Gene
    Calx (a.k.a. Dmel_CG5685, 9C5, CALX, CALX1.1, CG5685, CalX, Dmel/Ncx, Dmel\CG5685, NCX, Ncx, anon-93ABa, calX, calx)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-calx1 was a gift from Craig Montell (Addgene plasmid # 58697 ; http://n2t.net/addgene:58697 ; RRID:Addgene_58697)
  • For your References section:

    Light Activation, Adaptation, and Cell Survival Functions of the Na(+)/Ca(2+) Exchanger CalX. Wang T, Xu H, Oberwinkler J, Gu Y, Hardie RC, Montell C. Neuron 2005 Feb 3;45(3):367-78. 10.1016/j.neuron.2004.12.046 PubMed 15694324