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CMV-10-3xFLAG-LGP2-MIIa
(Plasmid #58684)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58684 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3X-FLAG-CMV-10
  • Backbone manufacturer
    Sigma Aldrich
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 8400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LGP2-MIIa
  • Alt name
    DHX58
  • Alt name
    LGP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2037
  • Mutation
    mutation to Motif IIA, K138E and Y142F
  • GenBank ID
    NM_024119
  • Entrez Gene
    DHX58 (a.k.a. D11LGP2, D11lgp2e, LGP2, RLR-3)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3x FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer CMV-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned in Horvath Lab by Akihiko Komuro, mutation by Annie Bruns

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-10-3xFLAG-LGP2-MIIa was a gift from Curt Horvath (Addgene plasmid # 58684 ; http://n2t.net/addgene:58684 ; RRID:Addgene_58684)

  • For your References section:

    ATP hydrolysis enhances RNA recognition and antiviral signal transduction by the innate immune sensor, laboratory of genetics and physiology 2 (LGP2). Bruns AM, Pollpeter D, Hadizadeh N, Myong S, Marko JF, Horvath CM. J Biol Chem. 2013 Jan 11;288(2):938-46. doi: 10.1074/jbc.M112.424416. Epub 2012 Nov 26. 10.1074/jbc.M112.424416 PubMed 23184951
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