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CMV-10-3xFLAG-LGP2-MIII
(Plasmid #58683)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58683 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3X-FLAG-CMV-10
  • Backbone manufacturer
    Sigma Aldrich
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 8400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LGP2-MIII
  • Alt name
    DHX58
  • Alt name
    LGP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2037
  • Mutation
    threonine and serine of helicase motif III mutated to alanine, T167A, S169A
  • GenBank ID
    NM_024119
  • Entrez Gene
    DHX58 (a.k.a. D11LGP2, D11lgp2e, LGP2, RLR-3)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3x FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer CMV-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned in Horvath Lab by Akihiko Komuro, mutation by Darja Pollpeter

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-10-3xFLAG-LGP2-MIII was a gift from Curt Horvath (Addgene plasmid # 58683 ; http://n2t.net/addgene:58683 ; RRID:Addgene_58683)

  • For your References section:

    Regulation of signal transduction by enzymatically inactive antiviral RNA helicase proteins MDA5, RIG-I, and LGP2. Bamming D, Horvath CM. J Biol Chem. 2009 Apr 10;284(15):9700-12. doi: 10.1074/jbc.M807365200. Epub 2009 Feb 11. 10.1074/jbc.M807365200 PubMed 19211564
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