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PurposeExpresses 3x Flag-tagged LGP2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3X-FLAG-CMV-10
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Backbone manufacturerSigma Aldrich
- Backbone size w/o insert (bp) 6300
- Total vector size (bp) 8400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namelaboratory of genetics and physiology 2
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Alt nameDHX58
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Alt nameLGP2
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SpeciesH. sapiens (human)
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Insert Size (bp)2037
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GenBank IDNM_024119
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Entrez GeneDHX58 (a.k.a. D11LGP2, D11lgp2e, LGP2, RLR-3)
- Promoter CMV
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Tag
/ Fusion Protein
- 3x FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer CMV-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned in Horvath Lab by Akihiko Komuro
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-10-3xFLAG-LGP2 was a gift from Curt Horvath (Addgene plasmid # 58681 ; http://n2t.net/addgene:58681 ; RRID:Addgene_58681) -
For your References section:
Regulation of signal transduction by enzymatically inactive antiviral RNA helicase proteins MDA5, RIG-I, and LGP2. Bamming D, Horvath CM. J Biol Chem. 2009 Apr 10;284(15):9700-12. doi: 10.1074/jbc.M807365200. Epub 2009 Feb 11. 10.1074/jbc.M807365200 PubMed 19211564