p41Nat MagicFluor
(Plasmid #58563)

• Purpose: p41Nat carrying both mating type markers buffered by the Cyc1 terminator
• Depositing Lab: Leonid Kruglyak
• Publication: Fluorescent X-QTL (unpublished)

Backbone

• Vector backbone: p41Nat 3-fragment GW
• Modifications to backbone: Insertion of Ste2p-Citrine, Cyc1 terminator and Ste3p-mcherry by Gateway cloning
• Vector type: Yeast Expression
• Selectable markers: clonNat

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 9329

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GGAAACAGCTATGACCATG

Resource Information

• Supplemental Documents:
  • GenBank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry