p41Nat 3-F GW
(Plasmid
#58553)
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Purpose(Empty Backbone) CEN vector with clonNat resistance marker for triple Gateway insertion
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS416
- Backbone size (bp) 7470
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Modifications to backboneInsertion of 3-fragment Gateway cassette and clonNat resistance gene
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Vector typeYeast Expression
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Selectable markersClonNat
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p41Nat 3-F GW was a gift from Leonid Kruglyak (Addgene plasmid # 58553 ; http://n2t.net/addgene:58553 ; RRID:Addgene_58553)