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PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58540 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneHSC1 retroviral vector
- Backbone size w/o insert (bp) 4941
- Total vector size (bp) 8805
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameEnhanced Green Fluorescence Protein, Puromycin resistance gene
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Alt nameEiP
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Insert Size (bp)1945
- Promoter Human EF1-alpha
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer ctcgatcctccctttatcca
- 3′ sequencing primer ccatatgatcgatgcatggg (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameCore HS4 insulator sequence (dimer)
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SpeciesG. gallus (chicken)
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Insert Size (bp)526
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer cccatgcatcgatcatatgg
- 3′ sequencing primer ctgttacccatgaaagaccc (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HSC1-HS4-GiP was a gift from James Ellis (Addgene plasmid # 58540 ; http://n2t.net/addgene:58540 ; RRID:Addgene_58540) -
For your References section:
Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element. Rival-Gervier S, Lo MY, Khattak S, Pasceri P, Lorincz MC, Ellis J. Mol Ther. 2013 Aug;21(8):1536-50. doi: 10.1038/mt.2013.131. Epub 2013 Jun 11. 10.1038/mt.2013.131 PubMed 23752310