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Addgene

pCAGGS-CD4-YEML-myc
(Plasmid #58538)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58538 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD4
  • Species
    H. sapiens (human)
  • Mutation
    Added C-terminal Tyrosine-Glutamate-Methionine-Leucine (YEML) motif followed by myc tag
  • Entrez Gene
    CD4 (a.k.a. CD4mut, IMD79, Leu-3, OKT4D, T4)
  • Tags / Fusion Proteins
    • IFITM3 endocytosis Tyrosine-Glutamate-Methionine-Leucine (YEML) motif (C terminal on insert)
    • myc (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Addgene's quality control sequencing has found an R318G amino acid residue substitution in CD4. The affect on protein function is not known.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-CD4-YEML-myc was a gift from Jacob Yount (Addgene plasmid # 58538 ; http://n2t.net/addgene:58538 ; RRID:Addgene_58538)

  • For your References section:

    Phosphorylation of the antiviral protein interferon-inducible transmembrane protein 3 (IFITM3) dually regulates its endocytosis and ubiquitination. Chesarino NM, McMichael TM, Hach JC, Yount JS. J Biol Chem. 2014 Apr 25;289(17):11986-92. doi: 10.1074/jbc.M114.557694. Epub 2014 Mar 13. 10.1074/jbc.M114.557694 PubMed 24627473
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