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PurposeExpresses human CD4 with a C-terminal myc tag in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58537 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD4
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SpeciesH. sapiens (human)
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Entrez GeneCD4 (a.k.a. CD4mut, IMD79, Leu-3, OKT4D, T4)
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Tag
/ Fusion Protein
- myc (C terminal on insert)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-CD4-myc was a gift from Jacob Yount (Addgene plasmid # 58537 ; http://n2t.net/addgene:58537 ; RRID:Addgene_58537) -
For your References section:
Phosphorylation of the antiviral protein interferon-inducible transmembrane protein 3 (IFITM3) dually regulates its endocytosis and ubiquitination. Chesarino NM, McMichael TM, Hach JC, Yount JS. J Biol Chem. 2014 Apr 25;289(17):11986-92. doi: 10.1074/jbc.M114.557694. Epub 2014 Mar 13. 10.1074/jbc.M114.557694 PubMed 24627473