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Addgene

p007ampGFP
(Plasmid #58535)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58535 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ401
  • Backbone size w/o insert (bp) 3979
  • Total vector size (bp) 4696
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Green fluorescent protein
  • Alt name
    GFP
  • Species
    Aquorea victoria
  • Insert Size (bp)
    717
  • Promoter T5 between two lac operators

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CTCGAAAATAATAAAGGGAAAATCAGT
  • 3′ sequencing primer CTCAGAAGTGAAACGCCGTA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DNA2.0, with GFP coming from another plasmid, pET EDNA
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternative name: p007-amp-GFP-T5lac Please visit https://www.biorxiv.org/content/10.1101/2021.06.20.449138v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p007ampGFP was a gift from Jaroslaw Bryk (Addgene plasmid # 58535 ; http://n2t.net/addgene:58535 ; RRID:Addgene_58535)

  • For your References section:

    UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene expression control and logic in E. coli. Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, Bryk J. bioRxiv 2021.06.20.449138 10.1101/2021.06.20.449138
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