pMaster3
(Plasmid #58526)

• Purpose: Reprogram somatic cells to iPS cells
• Depositing Lab: Mario Capecchi
• Publication: Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7.

Backbone

• Vector backbone: pCEP4
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: neo
• Insert Size (bp): 816

Gene/Insert 2

• Gene/Insert name: tk
• Insert Size (bp): 1131

Gene/Insert 3

• Gene/Insert name: oct4
• Species: H. sapiens (human)
• Insert Size (bp): 1317
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)

Gene/Insert 4

• Gene/Insert name: Klf4
• Species: H. sapiens (human)
• Insert Size (bp): 971
• Entrez Gene: KLF4 (a.k.a. EZF, GKLF)

Gene/Insert 5

• Gene/Insert name: Sox2
• Insert Size (bp): 1610
• Entrez Gene: SOX2 (a.k.a. ANOP3, MCOPS3)

Gene/Insert 6

• Gene/Insert name: myc
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Gene/Insert 7

• Gene/Insert name: EBNA-1
• Insert Size (bp): 1923

Gene/Insert 8

• Gene/Insert name: Amp
• Insert Size (bp): 858

Gene/Insert 9

• Gene/Insert name: Lin28
• Species: H. sapiens (human)
• Insert Size (bp): 2647
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)

Gene/Insert 10

• Gene/Insert name: Nanog
• Species: H. sapiens (human)
• Insert Size (bp): 935
• Entrez Gene: NANOG

Gene/Insert 11

• Gene/Insert name: IRES
• Insert Size (bp): 581

Gene/Insert 12

• Gene/Insert name: NR5A2
• Species: H. sapiens (human)
• Insert Size (bp): 1305
• Entrez Gene: NR5A2 (a.k.a. B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry