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pTRF.1-mCMV-udsVenus
(Plasmid #58518)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58518 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRF1
  • Backbone manufacturer
    System Bioscience
  • Backbone size w/o insert (bp) 5983
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pTRF.1-mCMV-udsVenus was constructed starting with the commercial lentiviral vector, pTRF1-mCMV-dscGFP (System Biosciences). Coral GFP (cGFP) was excised from pTRF1-mCMV-dscGFP by restriction digest with HindIII and EcoRV, and the vector was ligated with a similarly digested Venus prepared by PCR cloning designed to contain the appropriate motif for N-end rule degradation. The resulting pTRF.1-mCMV-dsVenus was then digested with HindIII, dephosphorylated and ligated with a similarly digested ubiquitin C monomer prepared by PCR cloning from oligo(dT)-primed MCF10A-5E cDNA to produce pTRF.1-mCMV-udsVenus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRF.1-mCMV-udsVenus was a gift from Kevin Janes (Addgene plasmid # 58518 ; http://n2t.net/addgene:58518 ; RRID:Addgene_58518)

  • For your References section:

    A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies. Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA. Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 2014 Mar 23. 10.1038/ncb2930 PubMed 24658685
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