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Addgene

pEN_TT JunD-HA
(Plasmid #58498)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58498 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEN_TTmiRc2
  • Backbone manufacturer
    Addgene #2752
  • Backbone size w/o insert (bp) 4422
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    JunD
  • Alt name
    jun D proto-oncogene
  • Alt name
    Jund
  • Alt name
    Jund1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1053
  • GenBank ID
    NM_010592
  • Entrez Gene
    Jund (a.k.a. Jund1)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site Mfe1 (not destroyed)
  • 5′ sequencing primer pCEP-fwd
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN_TT JunD-HA was a gift from Kevin Janes (Addgene plasmid # 58498 ; http://n2t.net/addgene:58498 ; RRID:Addgene_58498)

  • For your References section:

    A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies. Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA. Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 2014 Mar 23. 10.1038/ncb2930 PubMed 24658685
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