pBabe JunD-Venus puro
(Plasmid #58496)

• Purpose: mammalian expression of Venus-tagged mouse JunD
• Depositing Lab: Kevin Janes
• Publication: Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 2014 Mar 23.

Backbone

• Vector backbone: pBabe C-terminal Venus puro
• Backbone size w/o insert (bp): 5900
• Modifications to backbone: pBABE puro was cut by SALI and ligated with venus cut by SALI/XhoI. A new BglII site was inserted immediately downstream of the SalI site by PCR during cloning.
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: JunD
• Alt name: jun D proto-oncogene
• Alt name: Jund
• Alt name: Jund1
• Species: M. musculus (mouse)
• Insert Size (bp): 1076
• GenBank ID: NM_010592
• Entrez Gene: Jund (a.k.a. Jund1)
• Tag / Fusion Protein:
  • Venus (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBabe JunD_Venus puro was constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe C-terminal Venus puro. The PCR product was gel purified, digested with BamHI and EcoRI, and then ligated into pBabe c-terminal Venus puro, which had been digested with BamHI and EcoRI and then dephosphorylated.