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pBabe RFP1-Smad2 neo
(Plasmid #58491)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58491 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBabe N-terminal RFP neo
  • Backbone size w/o insert (bp) 5729
  • Modifications to backbone
    This construct is a variant of pBabe neo that expresses mRFP1 as an N-terminal fusion. A new XhoI site was inserted immediately upstream of the BamHI site by PCR during cloning. TO EXPRESS AN N-mRFP1 FUSION, GENES MUST HAVE A BAMHI- OR XHO1-COMPATIBLE 5' STICKY END.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad2
  • Alt name
    SMAD family member 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1414
  • GenBank ID
    NM_010754
  • Entrez Gene
    Smad2 (a.k.a. 7120426M23Rik, Madh2, Madr2, Smad-2, mMad2)
  • Tag / Fusion Protein
    • mRFP1 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBabe RFP1_Smad2 neo was constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe N-terminal RFP neo. The PCR product was gel purified, digested with BclI and SalI, and then ligated into pBabe N-terminal RFP neo, which had been digested with BamHI and SalI and then dephosphorylated.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe RFP1-Smad2 neo was a gift from Kevin Janes (Addgene plasmid # 58491 ; http://n2t.net/addgene:58491 ; RRID:Addgene_58491)
  • For your References section:

    A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies. Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA. Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 2014 Mar 23. 10.1038/ncb2930 PubMed 24658685