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pBabe JUND-HA neo
(Plasmid #58489)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58489 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe neo
  • Backbone manufacturer
    Weinberg Lab (Addgene plasmid 1767)
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    JUND
  • Alt name
    jun D proto-oncogene
  • Alt name
    AP-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1071
  • GenBank ID
    NM_005354
  • Entrez Gene
    JUN (a.k.a. AP-1, AP1, c-Jun, cJUN, p39)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBabe JUND_HA neo was constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe neo. The PCR product was gel purified, digested with BamHI and EcoRI, and then ligated into pBabe neo, which had been digested with BamHI and EcoRI and then dephosphorylated.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe JUND-HA neo was a gift from Kevin Janes (Addgene plasmid # 58489 ; http://n2t.net/addgene:58489 ; RRID:Addgene_58489)

  • For your References section:

    A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies. Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA. Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 2014 Mar 23. 10.1038/ncb2930 PubMed 24658685
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