pmCherry-C1 mCherry-NLS
(Plasmid #58476)

• Purpose: Expresses a nuclear-targeted mCherry control construct, on a CMV reporter
• Depositing Lab: Dyche Mullins
• Publication: Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07735.

Backbone

• Vector backbone: pmCherry-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 4788
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3X NLS with 3X stop codons
• Species: SV40 virus
• Insert Size (bp): 81
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: mCherry Forward
• 3′ sequencing primer: SV40 polyA Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: 3X NLS (with stop codons) was cloned by insertion of annealed primers with flanking restriction sites.
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry