pEGFP-C1 Lifeact-EGFP
(Plasmid #58470)

• Purpose: Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter
• Depositing Lab: Dyche Mullins
• Publication: Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 4755
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lifeact
• Species: Synthetic
• Insert Size (bp): 60
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: EGFP Reverse

Resource Information

• Addgene Notes:
  • Addgene Diagnosti Digest
• A portion of this plasmid was derived from a plasmid made by: Lifeact was cloned by insertion of annealed primers with flanking restriction sites.
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry