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Addgene

pEGFP-C1 Lifeact-EGFP-2XNLS
(Plasmid #58467)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58467 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Lifeact
  • Species
    Synthetic
  • Insert Size (bp)
    60
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer EGFP Reverse
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    2X NLS with 2X stop codons
  • Species
    SV40 virus
  • Insert Size (bp)
    72
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer EGFP Forward
  • 3′ sequencing primer SV40 polyA Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    3X NLS (with stop codons) and Lifeact were both cloned by insertion of annealed primers with flanking restriction sites.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1 Lifeact-EGFP-2XNLS was a gift from Dyche Mullins (Addgene plasmid # 58467 ; http://n2t.net/addgene:58467 ; RRID:Addgene_58467)
  • For your References section:

    Visualization of actin filaments and monomers in somatic cell nuclei. Belin BJ, Cimini BA, Blackburn EH, Mullins RD. Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. Epub 2013 Feb 27. 10.1091/mbc.E12-09-0685 PubMed 23447706