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Purposeexpression of mouse Ptc1-YFP
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEYFP-N3
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePtc1
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Alt namepatched1
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SpeciesM. musculus (mouse)
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Entrez GenePtch1 (a.k.a. A230106A15Rik, Ptc, Ptc1, Ptch, mes, wig)
- Promoter CMV
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Tag
/ Fusion Protein
- EYFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (destroyed during cloning)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ptc1-YFP was a gift from Matthew Scott (Addgene plasmid # 58456 ; http://n2t.net/addgene:58456 ; RRID:Addgene_58456) -
For your References section:
Patched1 regulates hedgehog signaling at the primary cilium. Rohatgi R, Milenkovic L, Scott MP. Science. 2007 Jul 20;317(5836):372-6. 10.1126/science.1139740 PubMed 17641202