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Addgene

pCMV-HA-mIFITM1-C49,50,83A
(Plasmid #58420)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58420 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-HA
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 3800
  • Total vector size (bp) 4118
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IFITM1
  • Species
    M. musculus (mouse)
  • Mutation
    Cysteines 49, 50, and 83 to Alanine
  • Entrez Gene
    Ifitm1 (a.k.a. 1110036C17Rik, DSPA2a, Mil-2, Mil2)
  • Promoter CMV IE
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GATCCGGTACTAGAGGAACTGAAAAAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-HA-mIFITM1-C49,50,83A was a gift from Jacob Yount (Addgene plasmid # 58420 ; http://n2t.net/addgene:58420 ; RRID:Addgene_58420)

  • For your References section:

    Palmitoylation on conserved and nonconserved cysteines of murine IFITM1 regulates its stability and anti-influenza A virus activity. Hach JC, McMichael T, Chesarino NM, Yount JS. J Virol. 2013 Sep;87(17):9923-7. doi: 10.1128/JVI.00621-13. Epub 2013 Jun 26. 10.1128/JVI.00621-13 PubMed 23804635
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