pCMV-HA-hIFITM1
(Plasmid #58399)

• Purpose: Expressed human IFITM1 with an N-terminal HA tag in mammalian cells
• Depositing Labs: Howard Hang, Jacob Yount
• Publication: Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi: 10.1038/nchembio.405. Epub 2010 Jul 4.

Backbone

• Vector backbone: pCMV-HA
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 3800
• Total vector size (bp): 4211
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IFITM1
• Species: H. sapiens (human)
• Entrez Gene: IFITM1 (a.k.a. 9-27, CD225, DSPA2a, IFI17, LEU13)
• Promoter: CMV IE
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GATCCGGTACTAGAGGAACTGAAAAAC

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry