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Addgene

pT2KXIGdeltaIn-MCS-birA-tagRFP
(Plasmid #58378)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58378 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pT2KXIGdeltaIn
  • Backbone manufacturer
    Kawakami
  • Backbone size w/o insert (bp) 6173
  • Total vector size (bp) 7883

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    birA tagRFP
  • Alt name
    biotin ligase
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1710
  • Tag / Fusion Protein
    • tagRFP (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Addgene's quality control sequencing has found some discrepancies with the depositor's uploaded sequence. The depositing lab is aware of these differences, and they are not thought to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2KXIGdeltaIn-MCS-birA-tagRFP was a gift from Didier Stainier (Addgene plasmid # 58378 ; http://n2t.net/addgene:58378 ; RRID:Addgene_58378)
  • For your References section:

    Translational profiling through biotinylation of tagged ribosomes in zebrafish. Housley MP, Reischauer S, Dieu M, Raes M, Stainier DY, Vanhollebeke B. Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub 2014 Sep 17. 10.1242/dev.111849 PubMed 25231762