pT2KXIGdeltaIn-MCS-birA-EGFP
(Plasmid #58377)

• Purpose: tol2 transgenesis for biotin based TRAP - BirA ligase
• Depositing Lab: Didier Stainier
• Publication: Housley et al Development. 2014 Oct;141(20):3988-93. doi: 10.1242/dev.111849. Epub 2014 Sep 17.

Backbone

• Vector backbone: pT2KXIGdeltaIn
• Backbone manufacturer: Kawakami
• Backbone size w/o insert (bp): 6208
• Total vector size (bp): 7903

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: birA EGFP
• Alt name: biotin ligase
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1695
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pT2KXIGdeltaIn.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Addgene's quality control sequencing has found some discrepancies with the depositor's uploaded sequence. The depositing lab is aware of these differences, and they are not thought to affect plasmid function.